The naturally sourced cyan blue colorant has colour properties that are nearly identical to those of the industry standard, brilliant blue FCF (FD&C Blue No. 1), an artificial colour that has been associated with negative health impacts.

The ingredient will meet growing consumer demands for more natural ingredients in food while keeping a vibrant colour palette, said the team of researchers.

Previous efforts to commercialise the blue pigment in red cabbage have been held back by the fact that natural blue colouring is present in tiny amounts in the vegetable that make it unviable.

Brassica Blue by Peak B

However, researchers at UC Davis, led by Pamela Denish, a graduate student at the UC Davis department of chemistry and Innovation Institute for Food and Health, have succeeded in designing an enzyme that converts purple and red anthocyanins in cabbage into blue.

The scientists screened public databases containing millions of enzymes for potential candidates and then tested a small number in the lab. based on those results, they used computational methods to search a huge number of potential protein sequences — 10 to the power of 20, more than the number of stars in the universe, they noted in a statement — to design an enzyme that would accomplish the conversion with high efficiency.

The blue colorant will be commercialized under the name ‘Brassica Blue’ by Peak B, a newly founded start-up located in Davis, California, which will be the exclusive licensee. The start-up has received angel funding and is fully equipped with laboratories and several outsourcing partners.

Production should begin in 2022 serving food industry customers first, followed by the pharmaceutical, textile, and cosmetic industries, said Pam Denish, the inventor and chief technology officer (CTO) of the start-up. Its CEO, Brock Siegal, has previous experience in the food industry and as a Silicon Valley executive.

Cheaper, more vibrant and more functional than natural equivalents

According to Denish, Brassica Blue has a clear competitive advantage over other natural blue colours, such as spirulina.

“Our blue colorant has performance and market advantages to spirulina,” she told The Ingredients Network. “Brassica Blue is a more vibrant cyan that is closer in hue to FD&C Blue 1, wheras spirulina is a paler blue. Spirulina is also hard to mix to make different colours. For example, in mixing with yellow to make green, Brassica Blue performs very well, but spirulina doesnt mix evenly.

“From a market perspective, Brassica Blue is cheaper to produce than spirulina colorant. Finally, spirulina is not abundant enough for global use by food manufacturers. The rarity of spirulina is not a problem while it is consumed primarily in the niche market. However, there would be a shortage were mainstream companies to try to incorporate it into their products.”

When asked whether the blue colour could be considered natural given that synthetic biology techniques are used in its manufacturing, Denish said the enzyme and biological components, apart from the red cabbage, are removed before final use.

“The synthetic biology techniques simply separate and enrich that compound from other components in red cabbage that interfere with the blue colour,” she said. “In other words, the only component actually used as colorant is natural. Similar processes are used in beer and cheese-making.”

‘Remarkable stability’

Brassica Blue shows good stability in product applications with no notable decay in colour when stored at ambient conditions over a 30-day period, according to the scientists. They tested it in a sugar syrup with a pH level of 7 for 55 days and reported a 14% loss of colour – “remarkable stability” compared to many naturally sourced colourants, including anthocyanins, they wrote in the study.

Nevertheless, more research is needed to evaluate the ingredient’s stability and colour vibrancy in a wide range of food and drink applications in order to determine usage limitations and appropriate safety precautions, they added.

Confectionery giant Mars, which funded the study, has tested the pigment in a blue ice cream.